Western blot analysis of extracts of various cell lines, using CLCN4 antibody (STJ115735) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CLCN4 antibody (600-700) (STJ115735)

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STJ115735
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLCN4
Gene ID: 1183
Uniprot ID: CLCN4_HUMAN
Immunogen Region: 600-700
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 600-700 of human CLCN4 (NP_001821.2).
Immunogen Sequence: MRPRRGEPPLSVLTQDSMTV EDVETLIKETDYNGFPVVVS RDSERLIGFAQRRELILAIK NARQRQEGIVSNSIMYFTEE PPELPANSPHPLKLRRILNL S
Tissue Specificity Abundant in skeletal muscle and also detectable in brain and heart.
Function Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters.
Protein Name H(+/Cl(- Exchange Transporter 4
Chloride Channel Protein 4
Clc-4
Chloride Transporter Clc-4
Database Links Reactome: R-HSA-2672351
Cellular Localisation Early Endosome Membrane
Multi-Pass Membrane Protein
Late Endosome Membrane
Endoplasmic Reticulum Membrane
Lysosome Membrane
Recycling Endosome Membrane
Localizes To Late Endosome Membrane
Lysosome Membrane And Recycling Endosome Membrane In The Presence Of Clcn3
Alternative Antibody Names Anti-H(+/Cl(- Exchange Transporter 4 antibody
Anti-Chloride Channel Protein 4 antibody
Anti-Clc-4 antibody
Anti-Chloride Transporter Clc-4 antibody
Anti-CLCN4 antibody

Information sourced from Uniprot.org

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