• Western blot analysis of extracts of A-375 cells, using CEP164 Antibody (STJ112005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using CEP164 antibody (STJ112005) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using CEP164 antibody (STJ112005) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat heart using CEP164 antibody (STJ112005) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat liver using CEP164 antibody (STJ112005) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat liver using CEP164 antibody (STJ112005) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using CEP164 antibody (STJ112005) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using CEP164 antibody (STJ112005) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver damage using CEP164 antibody (STJ112005) (40x lens).
  • Western blot analysis of extracts of A-375 cells, using CEP164 Antibody (STJ112005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CEP164 antibody (730-829) (STJ112005)

SKU:
STJ112005

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CEP164 (730-829) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CEP164
Gene ID: 22897
Uniprot ID: CE164_HUMAN
Immunogen Region: 730-829
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 730-829 of human CEP164 (NP_055771.4).
Immunogen Sequence: EASEKSEQAALNAAKEKALQ QLREQLEGERKEAVATLEKE HSAELERLCSSLEAKHREVV SSLQKKIQEAQQKEEAQLQK CLGQVEHRVHQKSYHVAGYE
Tissue Specificity Expressed in several cell lines.
Post Translational Modifications Phosphorylation at Ser-186 is induced upon DNA-damage caused by treatment with IR irradiation, UV irradiation, hydroxyurea or amphidicolin. Also MDC1-mediated chromatin remodeling is critical for DNA damage-induced phosphorylation.
Function Plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.
Protein Name Centrosomal Protein Of 164 Kda
Cep164
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Nucleus
Localizes Specifically To Very Distally Located Appendage Structures On The Mature Centriole From Which Initiate Pc Formation
Persisted At Centrioles Throughout Mitosis
Expressed In Chromatin-Enriched Nuclear Fraction Of Hela Cells
In Response To Dna Damage
It Translocates To Nuclear Foci That Contain The Dna Damage Response Proteins Kat5/Tip60 And Chek1
Alternative Antibody Names Anti-Centrosomal Protein Of 164 Kda antibody
Anti-Cep164 antibody
Anti-CEP164 antibody
Anti-KIAA1052 antibody
Anti-NPHP15 antibody

Information sourced from Uniprot.org

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