| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S5MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:6000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDK2 |
| Gene ID: | 1017 |
| Uniprot ID: | CDK2_HUMAN |
| Immunogen Region: | 199-298 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 199-298 of human CDK2 (P24941). |
| Immunogen Sequence: | RRALFPGDSEIDQLFRIFRT LGTPDEVVWPGVTSMPDYKP SFPKWARQDFSKVVPPLDED GRSLLSQMLHYDPNKRISAK AALAHPFFQDVTKPVPHLRL |
| Post Translational Modifications | Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A. Nitrosylated after treatment with nitric oxide (DETA-NO). |
| Function | Serine/threonine-protein kinase involved in the control of the cell cycle.essential for meiosis, but dispensable for mitosis. Phosphorylates CABLES1, CTNNB1, CDK2AP2, ERCC6, NBN, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression.controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in embryonic stem cells (ESCs). Activity of CDK2 is maximal during S phase and G2.activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis.regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Involved in regulation of telomere repair by mediating phosphorylation of NBN. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks. |
| Protein Name | Cyclin-Dependent Kinase 2 Cell Division Protein Kinase 2 P33 Protein Kinase |
| Database Links | Reactome: R-HSA-1538133 Reactome: R-HSA-171319 Reactome: R-HSA-176187 Reactome: R-HSA-176408 Reactome: R-HSA-187577 Reactome: R-HSA-2559582 Reactome: R-HSA-2559586 Reactome: R-HSA-5693607 Reactome: R-HSA-6804116 Reactome: R-HSA-6804756 Reactome: R-HSA-6804757 Reactome: R-HSA-68911 Reactome: R-HSA-68949 Reactome: R-HSA-68962 Reactome: R-HSA-69017 Reactome: R-HSA-69200 Reactome: R-HSA-69202 Reactome: R-HSA-69231 Reactome: R-HSA-69273 Reactome: R-HSA-69563 Reactome: R-HSA-69656 Reactome: R-HSA-8849470 Reactome: R-HSA-912446 Reactome: R-HSA-9616222 Reactome: R-HSA-9661069 Reactome: R-HSA-9825892 Reactome: R-HSA-983231 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Nucleus Cajal Body Endosome Localized At The Centrosomes In Late G2 Phase After Separation Of The Centrosomes But Before The Start Of Prophase Nuclear-Cytoplasmic Trafficking Is Mediated During The Inhibition By 1 25-(Oh)(2)D(3) |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 2 antibody Anti-Cell Division Protein Kinase 2 antibody Anti-P33 Protein Kinase antibody Anti-CDK2 antibody Anti-CDKN2 antibody |
Information sourced from Uniprot.org