| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CDC25B (1-250) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CDC25B |
| Gene ID: |
994 |
| Uniprot ID: |
MPIP2_HUMAN |
| Immunogen Region: |
1-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CDC25B (NP_068659.1). |
| Immunogen Sequence: |
MEVPQPEPAPGSALSPAGVC GGAQRPGHLPGLLLGSHGLL GSPVRAAASSPVTTLTQTMH DLAGLGSETPKSQVGTLLFR SRSRLTHLSLSRRASESSLS SESSESSDAGLCMDSPSPMD PHMAEQTFEQAIQAASRIIR NEQFAIRRFQSMPVRLLGHS PVLRNITNSQAPDGRRKSEA GSGAASSSGEDKENDGFVFK MPWKPTHPSSTHALAEWASR REAFAQRPSSAPDLMCLSP |
| Post Translational Modifications | Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. |
| Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. |
| Protein Name | M-Phase Inducer Phosphatase 2Dual Specificity Phosphatase Cdc25b |
| Database Links | Reactome: R-HSA-69273Reactome: R-HSA-69656Reactome: R-HSA-8862803 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle Pole |
| Alternative Antibody Names | Anti-M-Phase Inducer Phosphatase 2 antibodyAnti-Dual Specificity Phosphatase Cdc25b antibodyAnti-CDC25B antibodyAnti-CDC25HU2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
