| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-CD9 antigen (101-150 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC-P 1:100-300 ELISA 1:20000 IF 1:100-300 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD9 |
| Gene ID: | 928 |
| Uniprot ID: | CD9_HUMAN |
| Immunogen Region: | 101-150 aa |
| Specificity: | CD9 Polyclonal Antibody detects endogenous levels of CD9 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human CD9 at the amino acid range 101-150 |
| Post Translational Modifications | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. Palmitoylation by ZDHHC2 regulates CD9 expression, association with other tetraspanin family proteins and function in cell adhesion. |
| Function | Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Acts as a receptor for PSG17. Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. |
| Protein Name | Cd9 Antigen 5h9 Antigen Cell Growth-Inhibiting Gene 2 Protein Leukocyte Antigen Mic3 Motility-Related Protein Mrp-1 Tetraspanin-29 Tspan-29 P24 Cd Antigen Cd9 |
| Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-1300645 Reactome: R-HSA-5336415 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Membrane Secreted Extracellular Exosome Present At The Cell Surface Of Oocytes Accumulates In The Adhesion Area Between The Sperm And Egg Following Interaction Between Izumo1 And Its Receptor Izumo1r/Juno |
| Alternative Antibody Names | Anti-Cd9 Antigen antibody Anti-5h9 Antigen antibody Anti-Cell Growth-Inhibiting Gene 2 Protein antibody Anti-Leukocyte Antigen Mic3 antibody Anti-Motility-Related Protein antibody Anti-Mrp-1 antibody Anti-Tetraspanin-29 antibody Anti-Tspan-29 antibody Anti-P24 antibody Anti-Cd Antigen Cd9 antibody Anti-CD9 antibody Anti-MIC3 antibody Anti-TSPAN29 antibody Anti-GIG2 antibody |
Information sourced from Uniprot.org