| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD46 |
| Gene ID: | 4179 |
| Uniprot ID: | MCP_HUMAN |
| Immunogen Region: | 40-320 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human CD46 (NP_722548.1). |
| Immunogen Sequence: | TFEAMELIGKPKPYYEIGER VDYKCKKGYFYIPPLATHTI CDRNHTWLPVSDDACYRETC PYIRDPLNGQAVPANGTYEF GYQMHFICNEGYYLIGEEIL YCELKGSVAIWSGKPPICEK VLCTPPPKIKNGKHTFSEVE VFEYLDAVTYSCDPAPGPDP FSLIGESTIYCGDNSVWSRA APECKVVKCRFPVVENGKQI SGFGKKFYYKATVMFECDKG FYLDGSDTIVCDSNSTWDP |
| Tissue Specificity | Expressed by all cells except erythrocytes. |
| Post Translational Modifications | N-glycosylated on Asn-83.Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding. Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding. In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae.which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK. |
| Function | Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. (Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells. (Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3. (Microbial infection) Acts as a receptor for cultured Measles virus. (Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6. (Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes. |
| Protein Name | Membrane Cofactor Protein Tlx Trophoblast Leukocyte Common Antigen Cd Antigen Cd46 |
| Database Links | Reactome: R-HSA-977606 |
| Cellular Localisation | Cytoplasmic Vesicle Secretory Vesicle Acrosome Inner Membrane Single-Pass Type I Membrane Protein Inner Acrosomal Membrane Of Spermatozoa Internalized Upon Binding Of Measles Virus Herpesvirus 6 Or Neisseria Gonorrhoeae Which Results In An Increased Susceptibility Of Infected Cells To Complement-Mediated Injury In Cancer Cells Or Cells Infected By Neisseria Shedding Leads To A Soluble Peptide |
| Alternative Antibody Names | Anti-Membrane Cofactor Protein antibody Anti-Tlx antibody Anti-Trophoblast Leukocyte Common Antigen antibody Anti-Cd Antigen Cd46 antibody Anti-CD46 antibody Anti-MCP antibody Anti-MIC10 antibody |
Information sourced from Uniprot.org