| Host: | Mouse |
| Applications: | FC/IF/IHC |
| Reactivity: | CynomolguS.Monkey/Human/Mouse/Rhesus |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-CD100 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | EBS-CD-045 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1k |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fixed tissues has not been established to date |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: | SEMA4D |
| Gene ID: | 10507 |
| Uniprot ID: | SEM4D_HUMAN |
| Immunogen: | A BALB/c mouse was immunized with PHA stimulated PBL. Fusion partner: NS-1. |
| Tissue Specificity | Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. |
| Function | Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system.induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. |
| Protein Name | Semaphorin-4d A8 Bb18 Gr3 Cd Antigen Cd100 |
| Database Links | Reactome: R-HSA-416550 Reactome: R-HSA-416572 Reactome: R-HSA-416700 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Semaphorin-4d antibody Anti-A8 antibody Anti-Bb18 antibody Anti-Gr3 antibody Anti-Cd Antigen Cd100 antibody Anti-SEMA4D antibody Anti-C9orf164 antibody Anti-CD100 antibody Anti-SEMAJ antibody |
Information sourced from Uniprot.org