| Host: | Mouse |
| Applications: | WB/IF/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Caspase-8 is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 2G12 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:1000-2000 IHC 1:200-500 IF 1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CASP8 |
| Gene ID: | 841 |
| Uniprot ID: | CASP8_HUMAN |
| Specificity: | The antibody detects endogenous Caspase-8 protein. |
| Immunogen: | Recombinant Protein of Caspase-8 |
| Post Translational Modifications | Generation of the p10 and p18 subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. Phosphorylation on Tyr-380 by SRC is mediated by interaction with the SRC SH2 domain and does not affect dimerization or recruitment to the death-inducing signaling complex (DISC) but negatively regulates DISC-mediated processing and activation of CASP8, down-regulating its proapoptotic function. Phosphorylation on Tyr-380 also enhances localization to lamellipodia in migrating cells. (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP8 processing, preventing CASP8 activation and ability to mediate extrinsic apoptosis. (Microbial infection) Proteolytically cleaved by the cowpox virus CRMA death inhibitory protein. |
| Function | Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-324', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response. Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis. Initiates pyroptosis following inactivation of MAP3K7/TAK1. Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production. May participate in the Granzyme B (GZMB) cell death pathways. Cleaves PARP1 and PARP2. Independent of its protease activity, promotes cell migration following phosphorylation at Tyr-380. Isoform 5: Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex. Isoform 6: Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex. Isoform 7: Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex (Probable). Acts as an inhibitor of the caspase cascade. Isoform 8: Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex. |
| Protein Name | Caspase-8 Casp-8 Apoptotic Cysteine Protease Apoptotic Protease Mch-5 Cap4 Fadd-Homologous Ice/Ced-3-Like Protease Fadd-Like Ice Flice Ice-Like Apoptotic Protease 5 Mort1-Associated Ced-3 Homolog Mach Cleaved Into - Caspase-8 Subunit P18 - Caspase-8 Subunit P10 |
| Database Links | Reactome: R-HSA-111465 Reactome: R-HSA-140534 Reactome: R-HSA-168638 Reactome: R-HSA-2562578 Reactome: R-HSA-264870 Reactome: R-HSA-3371378 Reactome: R-HSA-5213460 Reactome: R-HSA-5218900 Reactome: R-HSA-5357786 Reactome: R-HSA-5357905 Reactome: R-HSA-5660668 Reactome: R-HSA-5675482 Reactome: R-HSA-69416 Reactome: R-HSA-75108 Reactome: R-HSA-75153 Reactome: R-HSA-75157 Reactome: R-HSA-75158 Reactome: R-HSA-9013957 Reactome: R-HSA-933543 Reactome: R-HSA-9686347 Reactome: R-HSA-9693928 Reactome: R-HSA-9758274 |
| Cellular Localisation | Cytoplasm Nucleus Cell Projection Lamellipodium Recruitment To Lamellipodia Of Migrating Cells Is Enhanced By Phosphorylation At Tyr-380 |
| Alternative Antibody Names | Anti-Caspase-8 antibody Anti-Casp-8 antibody Anti-Apoptotic Cysteine Protease antibody Anti-Apoptotic Protease Mch-5 antibody Anti-Cap4 antibody Anti-Fadd-Homologous Ice/Ced-3-Like Protease antibody Anti-Fadd-Like Ice antibody Anti-Flice antibody Anti-Ice-Like Apoptotic Protease 5 antibody Anti-Mort1-Associated Ced-3 Homolog antibody Anti-Mach Cleaved Into - Caspase-8 Subunit P18 - Caspase-8 Subunit P10 antibody Anti-CASP8 antibody Anti-MCH5 antibody |
Information sourced from Uniprot.org