Anti-Caldesmon (HMW) antibody [H-CALD] (STJ16101860)

SKU:
STJ16101860-1

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Host: Mouse
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Caldesmon (HMW) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: H-CALD
Conjugation: Unconjugated
Isotype: IgGk
Formulation: Bioreactor Concentrate with 0.05% Azide
Purification: Concentrate
Dilution Range: IHC-P 1:400-1:800
Storage Instruction: Store at 2-8°C upon receipt.
Immunogen: Crude human uterus extract
Background Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120–150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70–80kDa) is found in non-muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Uterus, blood cessels, smooth muscle or leiomyosarcoma. Staining Cytoplasmic

Information sourced from Uniprot.org

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