| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-C4A (1200-1446) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
C4A |
| Gene ID: |
720/721 |
| Uniprot ID: |
CO4A_HUMAN |
| Immunogen Region: |
1200-1446 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1446 of human Complement C4A (NP_009224.2). |
| Immunogen Sequence: |
LTLTKAPVDLLGVAHNNLMA MAQETGDNLYWGSVTGSQSN AVSPTPAPRNPSDPMPQAPA LWIETTAYALLHLLLHEGKA EMADQASAWLTRQGSFQGGF RSTQDTVIALDALSAYWIAS HTTEERGLNVTLSSTGRNGF KSHALQLNNRQIRGLEEELQ FSLGSKINVKVGGNSKGTLK VLRTYNVLDMKNTTCQDLQI EVTVKGHVEYTMEANEDYED YEYDELPAKDDPDAPLQPV |
| Tissue Specificity | Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland, and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra-hepatic sites of expression may be important for the local protection and inflammatory response. |
| Post Translational Modifications | Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
| Function | Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. |
| Protein Name | Complement C4-AAcidic Complement C4C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 2 Cleaved Into - Complement C4 Beta Chain - Complement C4-A Alpha Chain - C4a Anaphylatoxin - C4b-A - C4d-A - Complement C4 Gamma Chain |
| Database Links | Reactome: R-HSA-166663Reactome: R-HSA-174577Reactome: R-HSA-381426Reactome: R-HSA-8957275Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedSynapseCell ProjectionAxonDendrite |
| Alternative Antibody Names | Anti-Complement C4-A antibodyAnti-Acidic Complement C4 antibodyAnti-C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 2 Cleaved Into - Complement C4 Beta Chain - Complement C4-A Alpha Chain - C4a Anaphylatoxin - C4b-A - C4d-A - Complement C4 Gamma Chain antibodyAnti-C4A antibodyAnti-CO4 antibodyAnti-CPAMD2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
