| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BUB1B |
| Gene ID: | 701 |
| Uniprot ID: | BUB1B_HUMAN |
| Immunogen Region: | 160-400 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human BUB1B (NP_001202.4). |
| Immunogen Sequence: | EEYEARENFRKADAIFQEGI QQKAEPLERLQSQHRQFQAR VSRQTLLALEKEEEEEVFES SVPQRSTLAELKSKGKKTAR APIIRVGGALKAPSQNRGLQ NPFPQQMQNNSRITVFDENA DEASTAELSKPTVQPWIAPP MPRAKENELQAGPWNTGRSL EHRPRGNTASLIAVPAVLPS FTPYVEETAQQPVMTPCKIE PSINHILSTRKPGKEEGDPL QRVQSHQQASEEKKEKMMY |
| Tissue Specificity | Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. |
| Post Translational Modifications | Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610. Acetylation at Lys-250 regulates its degradation and timing in anaphase entry. Ubiquitinated. Degraded by the proteasome. Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex. Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore. Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase. |
| Function | Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. |
| Protein Name | Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1 Beta Mad3/Bub1-Related Protein Kinase Hbubr1 Mitotic Checkpoint Kinase Mad3l Protein Ssk1 |
| Database Links | Reactome: R-HSA-141430 Reactome: R-HSA-141444 Reactome: R-HSA-174184 Reactome: R-HSA-176409 Reactome: R-HSA-179409 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-5663220 Reactome: R-HSA-68877 Reactome: R-HSA-9648025 |
| Cellular Localisation | Cytoplasm Nucleus Chromosome Centromere Kinetochore Cytoskeleton Microtubule Organizing Center Centrosome Cytoplasmic In Interphase Cells Associates With The Kinetochores In Early Prophase Kinetochore Localization Requires Bub1 Plk1 And Knl1 |
| Alternative Antibody Names | Anti-Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1 Beta antibody Anti-Mad3/Bub1-Related Protein Kinase antibody Anti-Hbubr1 antibody Anti-Mitotic Checkpoint Kinase Mad3l antibody Anti-Protein Ssk1 antibody Anti-BUB1B antibody Anti-BUBR1 antibody Anti-MAD3L antibody Anti-SSK1 antibody |
Information sourced from Uniprot.org