| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BABAM2 |
| Gene ID: | 9577 |
| Uniprot ID: | BABA2_HUMAN |
| Immunogen Region: | 51-229 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 51-229 of human BRE (NP_954661.1). |
| Immunogen Sequence: | PNCDRFKLHIPYAGETLKWD IIFNAQYPELPPDFIFGEDA EFLPDPSALQNLASWNPSNP ECLLLVVKELVQQYHQFQCS RLRESSRLMFEYQTLLEEPQ YGENMEIYAGKKNNWTGEFS ARFLLKLPVDFSNIPTYLLK DVNEDPGEDVALLSVSFEDT EATQVYPKLYLSPRIEHAL |
| Tissue Specificity | Expressed in all cell lines examined. Highly expressed in placenta. |
| Function | Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Within the BRISC complex, acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. The BRISC complex plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1.deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A.however these effects may be indirect. |
| Protein Name | Brisc And Brca1-A Complex Member 2 Brca1-A Complex Subunit Bre Brca1/Brca2-Containing Complex Subunit 45 Brain And Reproductive Organ-Expressed Protein |
| Database Links | Reactome: R-HSA-5689901 Reactome: R-HSA-5693565 Reactome: R-HSA-5693571 Reactome: R-HSA-5693607 Reactome: R-HSA-69473 |
| Cellular Localisation | Cytoplasm Nucleus Localizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs) |
| Alternative Antibody Names | Anti-Brisc And Brca1-A Complex Member 2 antibody Anti-Brca1-A Complex Subunit Bre antibody Anti-Brca1/Brca2-Containing Complex Subunit 45 antibody Anti-Brain And Reproductive Organ-Expressed Protein antibody Anti-BABAM2 antibody Anti-BRCC45 antibody Anti-BRE antibody |
Information sourced from Uniprot.org