• Immunohistochemistry analysis of BOP1 in paraffin-embedded mouse brain using BOP1 Rabbit polyclonal antibody (STJ11103243) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BOP1 in paraffin-embedded rat lung using BOP1 Rabbit polyclonal antibody (STJ11103243) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BOP1 Rabbit polyclonal antibody (STJ11103243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BOP1 antibody (600-680) (STJ11103243)

SKU:
STJ11103243

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BOP1 (600-680) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BOP1
Gene ID: 23246
Uniprot ID: BOP1_HUMAN
Immunogen Region: 600-680
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 600-680 of human BOP1 (NP_056016.1).
Immunogen Sequence: RSVRLYHLLRQELTKKLMPN CKWVSSLAVHPAGDNVICGS YDSKLVWFDLDLSTKPYRML RHHKKALRAVAFHPRYPLFA S
Function Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Protein Name Ribosome Biogenesis Protein Bop1
Block Of Proliferation 1 Protein
Database Links Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Alternative Antibody Names Anti-Ribosome Biogenesis Protein Bop1 antibody
Anti-Block Of Proliferation 1 Protein antibody
Anti-BOP1 antibody
Anti-KIAA0124 antibody

Information sourced from Uniprot.org

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