| Host: | Mouse |
| Applications: | WB/IHC/IF |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Baculoviral IAP repeat-containing protein 5 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality : | Monoclonal |
| Clone ID : | Mix |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:1000-2000 IHC 1:200-500 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BIRC5 |
| Gene ID: | 332 |
| Uniprot ID: | BIRC5_HUMAN |
| Specificity: | The antibody detects endogenous Survivin protein. |
| Immunogen: | Recombinant Protein of Survivin |
| Post Translational Modifications | Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting. Deubiquitinated by USP35 or USP38.leading to stabilization. In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities. Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity. Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis. Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation. |
| Function | Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex.directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Essential for the maintenance of mitochondrial integrity and function. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. |
| Protein Name | Baculoviral Iap Repeat-Containing Protein 5 Apoptosis Inhibitor 4 Apoptosis Inhibitor Survivin |
| Database Links | Reactome: R-HSA-141444 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-4615885 Reactome: R-HSA-5663220 Reactome: R-HSA-6785807 Reactome: R-HSA-6803205 Reactome: R-HSA-68877 Reactome: R-HSA-8951664 Reactome: R-HSA-9648025 |
| Cellular Localisation | Cytoplasm Nucleus Chromosome Centromere Cytoskeleton Spindle Kinetochore Midbody Localizes At The Centromeres From Prophase To Metaphase At The Spindle Midzone During Anaphase And A The Midbody During Telophase And Cytokinesis Accumulates In The Nucleus Upon Treatment With Leptomycin B (Lmb) A Xpo1/Crm1 Nuclear Export Inhibitor Localizes On Chromosome Arms And Inner Centromeres From Prophase Through Metaphase Localizes To Kinetochores In Metaphase Distributes To The Midzone Microtubules In Anaphase And At Telophase Localizes Exclusively To The Midbody Colocalizes With Aurkb At Mitotic Chromosomes Acetylation At Lys-129 Directs Its Localization To The Nucleus By Enhancing Homodimerization And Thereby Inhibiting Xpo1/Crm1-Mediated Nuclear Export |
| Alternative Antibody Names | Anti-Baculoviral Iap Repeat-Containing Protein 5 antibody Anti-Apoptosis Inhibitor 4 antibody Anti-Apoptosis Inhibitor Survivin antibody Anti-BIRC5 antibody Anti-API4 antibody Anti-IAP4 antibody |
Information sourced from Uniprot.org