• Western blot analysis of extracts of various cell lines, using Biotin antibody (STJ11103412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Biotin antibody (STJ11103412)

SKU:
STJ11103412
Current Stock:
Host: Rabbit
Applications: WB
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-Biotin is suitable for use in Western Blot.
Clonality: Monoclonal
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Immunogen: Recombinant protein of human Biotin.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance