| Host: | Mouse |
| Applications: | WB/ICC/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-beta III Tubulin is suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence research applications. |
| Clonality : | Monoclonal |
| Clone ID : | SM0434 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol. |
| Purification: | Affinity purification |
| Dilution Range: | WB (H, M, R) 1:1000-1:2000 ICC/IF (M) 1:500-1:1000 |
| Storage Instruction: | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
| Gene Symbol: | Tubb3 |
| Gene ID: | 22152 |
| Uniprot ID: | TBB3_MOUSE |
| Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Beta 3-tubulin |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglycylated, resulting in polyglycine chains on the gamma-carboxyl group. Glycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering polyglycylation levels increases polyglutamylation, and reciprocally. Cilia and flagella glycylation is required for their stability and maintenance. Flagella glycylation controls sperm motility. Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. |
| Protein Name | Tubulin Beta-3 Chain |
| Database Links | Reactome: R-MMU-190840 Reactome: -MMU-2132295 Reactome: -MMU-2467813 Reactome: -MMU-2500257 Reactome: -MMU-3371497 Reactome: -MMU-380320 Reactome: -MMU-437239 Reactome: -MMU-5610787 Reactome: -MMU-5617833 Reactome: -MMU-5620924 Reactome: -MMU-5626467 Reactome: -MMU-5663220 Reactome: -MMU-6807878 Reactome: -MMU-6811434 Reactome: -MMU-6811436 Reactome: -MMU-68877 Reactome: -MMU-8852276 Reactome: -MMU-8955332 Reactome: -MMU-9646399 Reactome: -MMU-9648025 Reactome: -MMU-9668328 Reactome: -MMU-983189 Reactome: -MMU-9833482 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cell Projection Growth Cone Lamellipodium Filopodium |
| Alternative Antibody Names | Anti-Tubulin Beta-3 Chain antibody Anti-Tubb3 antibody |
Information sourced from Uniprot.org