| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BAX |
| Gene ID: | 581 |
| Uniprot ID: | BAX_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Bax (Q07812). |
| Immunogen Sequence: | MDGSGEQPRGGGPTSSEQIM KTGALLLQGFIQDRAGRMGG EAPELALDPVPQDASTKKLS ECLKRIGDELDSNMELQRMI AAVDTDSPREVFFRVAADMF |
| Tissue Specificity | Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. |
| Post Translational Modifications | Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12. |
| Function | Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
| Protein Name | Apoptosis Regulator Bax Bcl-2-Like Protein 4 Bcl2-L-4 |
| Database Links | Reactome: R-HSA-111457 Reactome: R-HSA-114294 Reactome: R-HSA-5620971 Reactome: R-HSA-6803204 Reactome: R-HSA-6804114 Reactome: R-HSA-8878166 Reactome: R-HSA-9603505 |
| Cellular Localisation | Isoform Alpha: Mitochondrion Outer Membrane Single-Pass Membrane Protein Cytoplasm Nucleus Colocalizes With 14-3-3 Proteins In The Cytoplasm Under Stress Conditions Undergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion Membrane Upon Sendai Virus Infection Recruited To The Mitochondrion Through Interaction With Irf3 Isoform Beta: Cytoplasm Isoform Gamma: Cytoplasm Isoform Delta: Cytoplasm |
| Alternative Antibody Names | Anti-Apoptosis Regulator Bax antibody Anti-Bcl-2-Like Protein 4 antibody Anti-Bcl2-L-4 antibody Anti-BAX antibody Anti-BCL2L4 antibody |
Information sourced from Uniprot.org