• Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using BAK1 Rabbit polyclonal antibody (STJ11104262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-BAK1 antibody (516-615) (STJ11104262)

SKU:
STJ11104262

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: A.thaliana
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BAK1 (516-615) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 516-615
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 516-615 of arabidopsis thaliana BAK1 (NP_567920.1).
Immunogen Sequence: KKLEALVDVDLQGNYKDEEV EQLIQVALLCTQSSPMERPK MSEVVRMLEGDGLAERWEEW QKEEMFRQDFNYPTHHPAVS GWIIGDSTSQIENEYPSGPR
Background Leu-rich receptor Serine/threonine protein kinase. Component of BR signaling that interacts with BRI1 in vitro and in vivo to form a heterodimer. Brassinolide-dependent association of BRI1 and BAK1 in vivo. Phosphorylation of both BRI1 and BAK1 on Thr residues was BR dependent. Although BAK1 and BRI1 alone localize in the plasma membrane, when BAK1 and BRI1 are coexpressed, the heterodimer BAK1/BRI1 they form is localized in the endosome.

Information sourced from Uniprot.org

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