• Immunofluorescence analysis of H9C2 cells using ATXN10 Rabbit polyclonal antibody (STJ22738) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ATXN10 antibody (STJ22738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATXN10 antibody (346-475) (STJ22738)

SKU:
STJ22738

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATXN10
Gene ID: 25814
Uniprot ID: ATX10_HUMAN
Immunogen Region: 346-475
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 346-475 of human ATXN10 (NP_037368.1).
Immunogen Sequence: ETTNIFSNCGCVRAEGDISN VANGFKSHLIRLIGNLCYKN KDNQDKVNELDGIPLILDNC NISDSNPFLTQWVIYAIRNL TEDNSQNQDLIAKMEEQGLA DASLLKKVGFEVEKKGEKLI LKSTRDTPKP
Tissue Specificity Expressed in the central nervous system.
Post Translational Modifications Polyubiquitinated. Phosphorylation at Ser-12 by AURKB promotes the association of ATXN10 with PLK1. Phosphorylation at Ser-77 and Thr-82 by PLK1 may play a role in the regulation of cytokinesis and may stimulate the proteasome-mediated degradation of ATXN10.
Function May play a role in the regulation of cytokinesis. May play a role in signaling by stimulating protein glycosylation. Induces neuritogenesis by activating the Ras-MAP kinase pathway and is necessary for the survival of cerebellar neurons. Does not appear to play a major role in ciliogenesis.
Protein Name Ataxin-10
Brain Protein E46 Homolog
Spinocerebellar Ataxia Type 10 Protein
Cellular Localisation Cytoplasm
Perinuclear Region
Midbody
Cytoskeleton
Cilium Basal Body
Microtubule Organizing Center
Centrosome
Centriole
Localizes To The Midbody During Telophase
Alternative Antibody Names Anti-Ataxin-10 antibody
Anti-Brain Protein E46 Homolog antibody
Anti-Spinocerebellar Ataxia Type 10 Protein antibody
Anti-ATXN10 antibody
Anti-SCA10 antibody

Information sourced from Uniprot.org

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