Western blot analysis of various lysates using ASPH Rabbit polyclonal antibody (STJ115119) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ASPH antibody (111-270) (STJ115119)

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STJ115119
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASPH
Gene ID: 444
Uniprot ID: ASPH_HUMAN
Immunogen Region: 111-270
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 111-270 of human ASPH (NP_001158227.1).
Immunogen Sequence: ERSTSEPAVPPEEAEPHTEP EEQVPVEAEPQNIEDEAKEQ IQSLLHEMVHAEHETEHSYH VEETVSQDCNQDMEEMMSEQ ENPDSSEPVVEDERLHHDTD DVTYQVYEEQAVYEPLENEG IEITEVTAPPEDNPVEDSQV IVEEVSIFPVEEQQEVPPDT
Tissue Specificity Isoform 1 is detected in all tissues tested. Isoform 8 is mainly expressed in pancreas, heart, brain, kidney and liver. Isoform 8 is expressed in kidney (at protein level).
Function Isoform 1: Specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. Isoform 8: Membrane-bound Ca(2+)-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) channels in T-cells.
Protein Name Aspartyl/Asparaginyl Beta-Hydroxylase
Aspartate Beta-Hydroxylase
Asp Beta-Hydroxylase
Peptide-Aspartate Beta-Dioxygenase
Database Links Reactome: R-HSA-2672351
Reactome: R-HSA-5578775
Reactome: R-HSA-9629569
Cellular Localisation Isoform 1: Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Isoform 4: Sarcoplasmic Reticulum Membrane
Isoform 8: Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Aspartyl/Asparaginyl Beta-Hydroxylase antibody
Anti-Aspartate Beta-Hydroxylase antibody
Anti-Asp Beta-Hydroxylase antibody
Anti-Peptide-Aspartate Beta-Dioxygenase antibody
Anti-ASPH antibody
Anti-BAH antibody

Information sourced from Uniprot.org

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