• Immunohistochemistry analysis of ARPC5L in paraffin-embedded Human lung cancer using ARPC5L Rabbit polyclonal antibody (STJ118349) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ARPC5L Rabbit polyclonal antibody (STJ118349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ARPC5L antibody (1-100) (STJ118349)

SKU:
STJ118349

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARPC5L (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARPC5L
Gene ID: 81873
Uniprot ID: ARP5L_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human ARPC5L (NP_112240.1).
Immunogen Sequence: MARNTLSSRFRRVDIDEFDE NKFVDEQEEAAAAAAEPGPD PSEVDGLLRQGDMLRAFHAA LRNSPVNTKNQAVKERAQGV VLKVLTNFKSSEIEQAVQSL
Function May function as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
Protein Name Actin-Related Protein 2/3 Complex Subunit 5-Like Protein
Arp2/3 Complex 16 Kda Subunit 2
Arc16-2
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Actin-Related Protein 2/3 Complex Subunit 5-Like Protein antibody
Anti-Arp2/3 Complex 16 Kda Subunit 2 antibody
Anti-Arc16-2 antibody
Anti-ARPC5L antibody

Information sourced from Uniprot.org

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