| Host: | Rabbit |
| Applications: | ELISA/IHC/IP/WB |
| Reactivity: | Human/Monkey/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-ANXA1 (1-50) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.55-0.75 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:100 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | ANXA1 |
| Gene ID: | 301 |
| Uniprot ID: | ANXA1_HUMAN |
| Immunogen Region: | 1-50 |
| Specificity: | This antibody will only detect Annexin A1 protein. The antibody will detect soluble Annexin A1. |
| Immunogen: | Synthetic peptide taken within amino acid region 1-50 on human annexin A1 protein. |
| Tissue Specificity | Detected in resting neutrophils. Detected in peripheral blood T-cells. Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors. Detected in placenta (at protein level). Detected in liver. |
| Post Translational Modifications | Phosphorylated by protein kinase C, EGFR and TRPM7. Phosphorylated in response to EGF treatment. Sumoylated. Proteolytically cleaved by cathepsin CTSG to release the active N-terminal peptide Ac2-26. |
| Function | Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions. Displays Ca(2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton. Annexin Ac2-26: Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2. |
| Protein Name | Annexin A1 Annexin I Annexin-1 Calpactin Ii Calpactin-2 Chromobindin-9 Lipocortin I Phospholipase A2 Inhibitory Protein P35 Cleaved Into - Annexin Ac2-26 |
| Database Links | Reactome: R-HSA-416476 Reactome: R-HSA-418594 Reactome: R-HSA-444473 Reactome: R-HSA-445355 Reactome: R-HSA-6785807 |
| Cellular Localisation | Nucleus Cytoplasm Cell Projection Cilium Cell Membrane Membrane Peripheral Membrane Protein Endosome Membrane Basolateral Cell Membrane Apical Cell Membrane Lateral Cell Membrane Secreted Extracellular Space Extracellular Side Extracellular Exosome Cytoplasmic Vesicle Secretory Vesicle Lumen Phagocytic Cup Early Endosome Cytoplasmic Vesicle Membrane At Least In Part Via Exosomes And Other Secretory Vesicles Detected In Exosomes And Other Extracellular Vesicles Alternatively The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 It Results In The Protein Translocation From The Cytoplasm Into Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion Detected In Gelatinase Granules In Resting Neutrophils Secretion Is Increased In Response To Wounding And Inflammation Secretion Is Increased Upon T-Cell Activation Neutrophil Adhesion To Endothelial Cells Stimulates Secretion Via Gelatinase Granules But Foreign Particle Phagocytosis Has No Effect Colocalizes With Actin Fibers At Phagocytic Cups Displays Calcium-Dependent Binding To Phospholipid Membranes |
| Alternative Antibody Names | Anti-Annexin A1 antibody Anti-Annexin I antibody Anti-Annexin-1 antibody Anti-Calpactin Ii antibody Anti-Calpactin-2 antibody Anti-Chromobindin-9 antibody Anti-Lipocortin I antibody Anti-Phospholipase A2 Inhibitory Protein antibody Anti-P35 Cleaved Into - Annexin Ac2-26 antibody Anti-ANXA1 antibody Anti-ANX1 antibody Anti-LPC1 antibody |
Information sourced from Uniprot.org