• Immunohistochemistry of baboon spleen with Anti-ANXA1 primary antibody (STJ500090) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ANXA1 antibody (1-50) (STJ500090)

SKU:
STJ500090-100

£441.50
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Host: Rabbit
Applications: ELISA/IHC/IP/WB
Reactivity: Human/Monkey/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANXA1 (1-50) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.55-0.75 µg/µl
Dilution Range: WB: 1:500
DB: 1:10, 000
ELISA: 1:10, 000
IP: 1:200
IHC: 1:100
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: ANXA1
Gene ID: 301
Uniprot ID: ANXA1_HUMAN
Immunogen Region: 1-50
Specificity: This antibody will only detect Annexin A1 protein. The antibody will detect soluble Annexin A1.
Immunogen: Synthetic peptide taken within amino acid region 1-50 on human annexin A1 protein.
Tissue Specificity Detected in resting neutrophils. Detected in peripheral blood T-cells. Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors. Detected in placenta (at protein level). Detected in liver.
Post Translational Modifications Phosphorylated by protein kinase C, EGFR and TRPM7. Phosphorylated in response to EGF treatment. Sumoylated. Proteolytically cleaved by cathepsin CTSG to release the active N-terminal peptide Ac2-26.
Function Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions. Displays Ca(2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton. Annexin Ac2-26: Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2.
Protein Name Annexin A1
Annexin I
Annexin-1
Calpactin Ii
Calpactin-2
Chromobindin-9
Lipocortin I
Phospholipase A2 Inhibitory Protein
P35 Cleaved Into - Annexin Ac2-26
Database Links Reactome: R-HSA-416476
Reactome: R-HSA-418594
Reactome: R-HSA-444473
Reactome: R-HSA-445355
Reactome: R-HSA-6785807
Cellular Localisation Nucleus
Cytoplasm
Cell Projection
Cilium
Cell Membrane
Membrane
Peripheral Membrane Protein
Endosome Membrane
Basolateral Cell Membrane
Apical Cell Membrane
Lateral Cell Membrane
Secreted
Extracellular Space
Extracellular Side
Extracellular Exosome
Cytoplasmic Vesicle
Secretory Vesicle Lumen
Phagocytic Cup
Early Endosome
Cytoplasmic Vesicle Membrane
At Least In Part Via Exosomes And Other Secretory Vesicles
Detected In Exosomes And Other Extracellular Vesicles
Alternatively
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In The Protein Translocation From The Cytoplasm Into Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Detected In Gelatinase Granules In Resting Neutrophils
Secretion Is Increased In Response To Wounding And Inflammation
Secretion Is Increased Upon T-Cell Activation
Neutrophil Adhesion To Endothelial Cells Stimulates Secretion Via Gelatinase Granules
But Foreign Particle Phagocytosis Has No Effect
Colocalizes With Actin Fibers At Phagocytic Cups
Displays Calcium-Dependent Binding To Phospholipid Membranes
Alternative Antibody Names Anti-Annexin A1 antibody
Anti-Annexin I antibody
Anti-Annexin-1 antibody
Anti-Calpactin Ii antibody
Anti-Calpactin-2 antibody
Anti-Chromobindin-9 antibody
Anti-Lipocortin I antibody
Anti-Phospholipase A2 Inhibitory Protein antibody
Anti-P35 Cleaved Into - Annexin Ac2-26 antibody
Anti-ANXA1 antibody
Anti-ANX1 antibody
Anti-LPC1 antibody

Information sourced from Uniprot.org

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