• Immunofluorescence analysis of NIH-3T3 cells using ALDH1A2 antibody (STJ29639) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ALDH1A2 antibody (STJ29639) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ALDH1A2 antibody (STJ29639) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ALDH1A2 antibody (STJ29639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALDH1A2 antibody (1-180) (STJ29639)

SKU:
STJ29639

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALDH1A2 (1-180) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDH1A2
Gene ID: 8854
Uniprot ID: AL1A2_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALDH1A2 (NP_733797.1).
Immunogen Sequence: MTSSKIEMPGEVKADPAALM ASLHLLPSPTPNLEIKYTKI FINNEWQNSESGRVFPVYNP ATGEQVCEVQEADKADIDKA VQAARLAFSLGSVWRRMDAS ERGRLLDKLADLVERDRAVL ATMESLNGGKPFLQAFYVDL QGVIKTFRYYAGWADKIHGM TIPVDGDYFTFTRHEPIGVC
Function Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively. Retinoate signaling is critical for the transcriptional control of many genes, for instance it is crucial for initiation of meiosis in both male and female (Probable). Recognizes retinal as substrate, both in its free form and when bound to cellular retinol-binding protein. Can metabolize octanal and decanal, but has only very low activity with benzaldehyde, acetaldehyde and propanal. Displays complete lack of activity with citral.
Protein Name Retinal Dehydrogenase 2
Raldh 2
Raldh2
Aldehyde Dehydrogenase Family 1 Member A2
Aldh1a2
Retinaldehyde-Specific Dehydrogenase Type 2
Raldh(Ii
Database Links Reactome: R-HSA-5365859
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Retinal Dehydrogenase 2 antibody
Anti-Raldh 2 antibody
Anti-Raldh2 antibody
Anti-Aldehyde Dehydrogenase Family 1 Member A2 antibody
Anti-Aldh1a2 antibody
Anti-Retinaldehyde-Specific Dehydrogenase Type 2 antibody
Anti-Raldh(Ii antibody
Anti-ALDH1A2 antibody
Anti-RALDH2 antibody

Information sourced from Uniprot.org

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