Western blot analysis of extracts of various cell lines, using AKR1D1 antibody (STJ111755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKR1D1 antibody (1-326) (STJ111755)

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STJ111755
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKR1D1
Gene ID: 6718
Uniprot ID: AK1D1_HUMAN
Immunogen Region: 1-326
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human AKR1D1 (NP_005980.1).
Immunogen Sequence: MDLSAASHRIPLSDGNSIPI IGLGTYSEPKSTPKGACATS VKVAIDTGYRHIDGAYIYQN EHEVGEAIREKIAEGKVRRE DIFYCGKLWATNHVPEMVRP TLERTLRVLQLDYVDLYIIE VPMAFKPGDEIYPRDENGKW LYHKSNLCATWEAMEACKDA GLVKSLGVSNFNRRQLELIL NKPGLKHKPVSNQVECHPYF TQPKLLKFCQQHDIVITAYS PLGTSRNPIWVNVSSPPLL
Tissue Specificity Highly expressed in liver. Expressed in testis and weakly in colon.
Function Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-(4)-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one).
Protein Name Aldo-Keto Reductase Family 1 Member D1
3-Oxo-5-Beta-Steroid 4-Dehydrogenase
Delta(4-3-Ketosteroid 5-Beta-Reductase
Delta(4-3-Oxosteroid 5-Beta-Reductase
Database Links Reactome: R-HSA-193368
Reactome: R-HSA-193775
Reactome: R-HSA-193807
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Aldo-Keto Reductase Family 1 Member D1 antibody
Anti-3-Oxo-5-Beta-Steroid 4-Dehydrogenase antibody
Anti-Delta(4-3-Ketosteroid 5-Beta-Reductase antibody
Anti-Delta(4-3-Oxosteroid 5-Beta-Reductase antibody
Anti-AKR1D1 antibody
Anti-SRD5B1 antibody

Information sourced from Uniprot.org

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