• Western blot analysis of extracts of various cell lines, using AIFM3 antibody (STJ111329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using AIFM3 antibody (STJ111329) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat kidney using AIFM3 antibody (STJ111329) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using AIFM3 antibody (STJ111329) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using AIFM3 antibody (STJ111329) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using AIFM3 antibody (STJ111329) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using AIFM3 antibody (STJ111329) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using AIFM3 antibody (STJ111329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AIFM3 antibody (1-200) (STJ111329)

SKU:
STJ111329

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AIFM3 (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AIFM3
Gene ID: 150209
Uniprot ID: AIFM3_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human AIFM3 (NP_001018070.1).
Immunogen Sequence: MGGCFSKPKPVELKIEVVLP EKERGKEELSASGKGSPRAY QGNGTARHFHTEERLSTPHP YPSPQDCVEAAVCHVKDLEN GQMREVELGWGKVLLVKDNG EFHALGHKCPHYGAPLVKGV LSRGRVRCPWHGACFNISTG DLEDFPGLDSLHKFQVKIEK EKVYVRASKQALQLQRRTKV MAKCISPSAGYSSSTNVLIV
Tissue Specificity Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).
Function Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.
Protein Name Apoptosis-Inducing Factor 3
Apoptosis-Inducing Factor-Like Protein
Cellular Localisation Mitochondrion
Does Not Translocate To The Nucleus Upon Induction Of Apoptosis
Alternative Antibody Names Anti-Apoptosis-Inducing Factor 3 antibody
Anti-Apoptosis-Inducing Factor-Like Protein antibody
Anti-AIFM3 antibody
Anti-AIFL antibody

Information sourced from Uniprot.org

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