Western blot analysis of extracts of various cell lines, using AGL antibody (STJ112091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AGL antibody (1233-1532) (STJ112091)

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STJ112091
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AGL
Gene ID: 178
Uniprot ID: GDE_HUMAN
Immunogen Region: 1233-1532
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1233-1532 of human AGL (NP_000633.2).
Immunogen Sequence: EGFNITAGVDEETGFVYGGN RFNCGTWMDKMGESDRARNR GIPATPRDGSAVEIVGLSKS AVRWLLELSKKNIFPYHEVT VKRHGKAIKVSYDEWNRKIQ DNFEKLFHVSEDPSDLNEKH PNLVHKRGIYKDSYGASSPW CDYQLRPNFTIAMVVAPELF TTEKAWKALEIAEKKLLGPL GMKTLDPDDMVYCGIYDNAL DNDNYNLAKGFNYHQGPEWL WPIGYFLRAKLYFSRLMGP
Tissue Specificity Liver, kidney and lymphoblastoid cells express predominantly isoform 1.whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle.
Post Translational Modifications The N-terminus is blocked. Ubiquitinated.
Function Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
Protein Name Glycogen Debranching Enzyme
Glycogen Debrancher Includes - 4-Alpha-Glucanotransferase
Oligo-1 -4-1 -4-Glucantransferase - Amylo-Alpha-1 -6-Glucosidase
Amylo-1 -6-Glucosidase
Dextrin 6-Alpha-D-Glucosidase
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-70221
Cellular Localisation Cytoplasm
Under Glycogenolytic Conditions Localizes To The Nucleus
Alternative Antibody Names Anti-Glycogen Debranching Enzyme antibody
Anti-Glycogen Debrancher Includes - 4-Alpha-Glucanotransferase antibody
Anti-Oligo-1 -4-1 -4-Glucantransferase - Amylo-Alpha-1 -6-Glucosidase antibody
Anti-Amylo-1 -6-Glucosidase antibody
Anti-Dextrin 6-Alpha-D-Glucosidase antibody
Anti-AGL antibody
Anti-GDE antibody

Information sourced from Uniprot.org

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