| Host: | Rabbit |
| Applications: | ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-ACKR2 (200-300) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.55-0.75 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | ACKR2 |
| Gene ID: | 1238 |
| Uniprot ID: | ACKR2_HUMAN |
| Immunogen Region: | 200-300 |
| Immunogen: | Synthetic peptide taken within amino acid region 200-300 on ACRD6 protein. |
| Tissue Specificity | Found in endothelial cells lining afferent lymphatics in dermis and lymph nodes. Also found in lymph nodes subcapsular and medullary sinuses, tonsillar lymphatic sinuses and lymphatics in mucosa and submucosa of small and large intestine and appendix. Also found in some malignant vascular tumors. Expressed at high levels in Kaposi sarcoma-related pathologies. Expressed on apoptotic neutrophils (at protein level). Expressed primarily in placenta and fetal liver, and found at very low levels in the lung and lymph node. |
| Post Translational Modifications | Phosphorylated on serine residues in the C-terminal cytoplasmic tail. |
| Function | Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling pathway. Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. Plays a major role in the immune silencing of macrophages during the resolution of inflammation. Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs. |
| Protein Name | Atypical Chemokine Receptor 2 C-C Chemokine Receptor D6 Chemokine Receptor Ccr-10 Chemokine Receptor Ccr-9 Chemokine-Binding Protein 2 Chemokine-Binding Protein D6 |
| Database Links | Reactome: R-HSA-380108 |
| Cellular Localisation | Early Endosome Recycling Endosome Cell Membrane Multi-Pass Membrane Protein Predominantly Localizes To Endocytic Vesicles And Upon Stimulation By The Ligand Is Internalized Via Clathrin-Coated Pits Once Internalized The Ligand Dissociates From The Receptor And Is Targeted To Degradation While The Receptor Is Recycled Back To The Cell Membrane |
| Alternative Antibody Names | Anti-Atypical Chemokine Receptor 2 antibody Anti-C-C Chemokine Receptor D6 antibody Anti-Chemokine Receptor Ccr-10 antibody Anti-Chemokine Receptor Ccr-9 antibody Anti-Chemokine-Binding Protein 2 antibody Anti-Chemokine-Binding Protein D6 antibody Anti-ACKR2 antibody Anti-CCBP2 antibody Anti-CCR10 antibody Anti-CMKBR9 antibody Anti-D6 antibody |
Information sourced from Uniprot.org