• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 (Acetyl-Lys27) Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys27) Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Acetyl-Histone H3 (K27) Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000
  • Western blot analysis of Hela cells using Acetyl-Histone H3 (K27) Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000
  • Immunofluorescence analysis of Hela cell. 1, Histone H3 (Acetyl Lys27) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H3-Lys27 antibody (10-90) (STJ90125)

SKU:
STJ90125

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ICC, IP, ELISA
Reactivity: Human, Mouse, Rat, Monkey
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H3.1/Histone H3.2/Histone H3.3-Lys27 (10-90) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
IP 1:200
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H3C15.H3C14.H3C13
H3-3A.H3-3B
126961/333932/653604
3020/3021
H32_HUMAN
H33_HUMAN
H31_HUMAN
Immunogen Region: 10-90
Specificity: Acetyl-Histone H3-Lys27 polyclonal antibody (Histone H3.1 and Histone H3.2 and Histone H3.3) binds to endogenous Histone H3.1 and Histone H3.2 and Histone H3.3 at the amino acid region 10-90.
Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys27 at amino acid range 11-60
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name Histone H3.1
Histone H3/A
Histone H3/B
Histone H3/C
Histone H3/D
Histone H3/F
Histone H3/H
Histone H3/I
Histone H3/J
Histone H3/K
Histone H3/L
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-1912408
Reactome: R-HSA-201722
Reactome: R-HSA-212300
Reactome: R-HSA-2299718
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-3214815
Reactome: R-HSA-3214841
Reactome: R-HSA-3214842
Reactome: R-HSA-3214847
Reactome: R-HSA-3214858
Reactome: R-HSA-3247509
Reactome: R-HSA-427359
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-5334118
Reactome: R-HSA-5578749
Reactome: R-HSA-5617472
Reactome: R-HSA-5625886
Reactome: R-HSA-73728
Reactome: R-HSA-73772
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-912446
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9616222
Reactome: R-HSA-977225
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone H3.1 antibody
Anti-Histone H3/A antibody
Anti-Histone H3/B antibody
Anti-Histone H3/C antibody
Anti-Histone H3/D antibody
Anti-Histone H3/F antibody
Anti-Histone H3/H antibody
Anti-Histone H3/I antibody
Anti-Histone H3/J antibody
Anti-Histone H3/K antibody
Anti-Histone H3/L antibody
Anti-H3C1 antibody
Anti-H3FA antibody
Anti-HIST1H3A.H3C2 antibody
Anti-H3FL antibody
Anti-HIST1H3B.H3C3 antibody
Anti-H3FC antibody
Anti-HIST1H3C.H3C4 antibody
Anti-H3FB antibody
Anti-HIST1H3D.H3C6 antibody
Anti-H3FD antibody
Anti-HIST1H3E.H3C7 antibody
Anti-H3FI antibody
Anti-HIST1H3F.H3C8 antibody
Anti-H3FH antibody
Anti-HIST1H3G.H3C10 antibody
Anti-H3FK antibody
Anti-HIST1H3H.H3C11 antibody
Anti-H3FF antibody
Anti-HIST1H3I.H3C12 antibody
Anti-H3FJ antibody
Anti-HIST1H3J antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance