| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ABCD4 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 70kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 68.6kDa according to NP_005041.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ABCD4 |
| Gene ID: | 5826 |
| Uniprot ID: | ABCD4_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_005041.1 |
| Specificity: | Reported variants represent identical protein: NP_064719.1 NP_064731.1 NP_064720.1 NP_064730.1 |
| Immunogen Sequence: | RDDIDNPDQRISQD |
| Function | Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner. Targeted by LMBRD1 lysosomal chaperone from the endoplasmic reticulum to the lysosomal membrane. Then forms a complex with lysosomal chaperone LMBRD1 and cytosolic MMACHC to transport cobalamin across the lysosomal membrane. |
| Protein Name | Lysosomal Cobalamin Transporter Abcd4Atp-Binding Cassette Sub-Family D Member 4Pmp70-Related ProteinP70rPeroxisomal Membrane Protein 1-LikePxmp1-LPeroxisomal Membrane Protein 69Pmp69 |
| Database Links | Reactome: R-HSA-5683329Reactome: R-HSA-9758881Reactome: R-HSA-9758890 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinLysosome MembraneTargeted By Lmbrd1 Lysosomal Chaperone To The Lysosomal Membrane |
| Alternative Antibody Names | Anti-Lysosomal Cobalamin Transporter Abcd4 antibodyAnti-Atp-Binding Cassette Sub-Family D Member 4 antibodyAnti-Pmp70-Related Protein antibodyAnti-P70r antibodyAnti-Peroxisomal Membrane Protein 1-Like antibodyAnti-Pxmp1-L antibodyAnti-Peroxisomal Membrane Protein 69 antibodyAnti-Pmp69 antibodyAnti-ABCD4 antibodyAnti-PXMP1L antibody |
Information sourced from Uniprot.org