||This SARS-CoV-2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
||FOR RESEARCH USE ONLY (RUO).
||Serum, plasma and other biological fluids
| Background || This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SARS-CoV-2 SARS-CoV-2. Samples (or Standards) and biotinylated detection antibody specific for Human CLU are added to the micro ELISA plate wells. Human CLU would combined with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of SARS-CoV-2 SARS-CoV-2. You can calculate the concentration of Human CLU in the samples by comparing the OD of the samples to the standard curve. |
Information sourced from Uniprot.org
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