| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This Spp1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Spp1 |
| Gene ID: |
25353 |
| Uniprot ID: |
OSTP_RAT |
| Specificity: |
This kit recognizes Rat OPN in samples. No significant cross-reactivity or interference between Rat OPN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | OsteopontinBone Sialoprotein 1Secreted Phosphoprotein 1Spp-1 |
| Database Links | Reactome: R-RNO-1474228Reactome: -RNO-186797Reactome: -RNO-216083Reactome: -RNO-381426Reactome: -RNO-8957275 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Osteopontin ELISA kitBone Sialoprotein 1 ELISA kitSecreted Phosphoprotein 1 ELISA kitSpp-1 ELISA kitSpp1 ELISA kit2b7 ELISA kitSpp-1 ELISA kit |
| Specificity | This kit recognizes Rat OPN in samples. No significant cross-reactivity or interference between Rat OPN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-108 | 98 |
| EDTA plasma (n=8) | 83-95 | 90 |
| Cell culture media (n=8) | 92-104 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.92 | 14.49 | 41.70 | 4.60 | 13.64 | 44.23 |
| Standard deviation | 0.26 | 0.70 | 1.53 | 0.30 | 0.74 | 2.28 |
| CV (%) | 5.28 | 4.83 | 3.67 | 6.52 | 5.43 | 5.15 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
