Protein Name | Placenta Growth FactorPlgf |
Database Links | Reactome: R-RNO-194313Reactome: -RNO-195399 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Placenta Growth Factor ELISA kitPlgf ELISA kitPgf ELISA kitPlgf ELISA kit |
Specificity | This kit recognizes Rat PGF in samples. No significant cross-reactivity or interference between Rat PGF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 88-102 | 94 |
EDTA plasma (n=8) | 95-109 | 101 |
Cell culture media (n=8) | 94-111 | 102 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 24.51 | 75.11 | 187.38 | 26.60 | 81.13 | 201.63 |
Standard deviation | 1.69 | 4.27 | 7.46 | 1.85 | 4.84 | 7.04 |
CV (%) | 6.90 | 5.68 | 3.98 | 6.95 | 5.97 | 3.49 |