Rat IL-1R2 (Interleukin 1 Receptor Type 2) Sandwich ELISA Kit (STJE0019802)

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STJE0019802
£163.50 - £357.50
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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description : This IL-1R2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 6 months. Following first use any unused wells and reagents should be stored according to the components table in the manual.
Assay Time: 1.5h
Detection: Colormetric
Gene Symbol: Il1r2
Gene ID: 117022
Uniprot ID: IL1R2_RAT
Specificity: This kit recognizes Rat IL-1R2 in samples. No significant crossreactivity or interference between Rat IL-1R2 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Post Translational Modifications A soluble form (sIL1R2) can also be produced by proteolytic cleavage at the cell surface (shedding) involving a metalloproteinase.
Function Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity.this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Protein Name Interleukin-1 Receptor Type 2
Il-1r-2
Il-1rt-2
Il-1rt2
Cd121 Antigen-Like Family Member B
Il-1 Type Ii Receptor
Interleukin-1 Receptor Beta
Il-1r-Beta
Interleukin-1 Receptor Type Ii
Cd Antigen Cd121b Cleaved Into - Interleukin-1 Receptor Type 2 - Membrane Form
Mil-1r2
Mil-1rii - Interleukin-1 Receptor Type 2 - Soluble Form
Sil-1r2
Sil-1rii
Database Links Reactome: R-RNO-9020702
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cell Membrane
Secreted
Alternative ELISA Names Interleukin-1 Receptor Type 2 ELISA kit
Il-1r-2 ELISA kit
Il-1rt-2 ELISA kit
Il-1rt2 ELISA kit
Cd121 Antigen-Like Family Member B ELISA kit
Il-1 Type Ii Receptor ELISA kit
Interleukin-1 Receptor Beta ELISA kit
Il-1r-Beta ELISA kit
Interleukin-1 Receptor Type Ii ELISA kit
Cd Antigen Cd121b Cleaved Into - Interleukin-1 Receptor Type 2 - Membrane Form ELISA kit
Mil-1r2 ELISA kit
Mil-1rii - Interleukin-1 Receptor Type 2 - Soluble Form ELISA kit
Sil-1r2 ELISA kit
Sil-1rii ELISA kit
Il1r2 ELISA kit
Il-1r2 ELISA kit
Il1rb ELISA kit
Specificity This kit recognizes IL-1R2 in samples. No significant cross-reactivity or interference between IL-1R2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T 2-8℃, 1 months
Reference Standard 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials 2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab (100×) 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL 2-8℃ (Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL96T*5: 5 vials, 20 mL 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL96T*5: 5 vials, 14 mL
Concentrated Wash Buffer (25×) 96T/48T/24T: 1 vial, 30 mL96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃ (Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-105 98
EDTA plasma(n=8) 91-106 97
Cell culture media(n=8) 93-108 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 103.86 300.76 873.21 107.58 317.91 824.64
Standard deviation 6.57 16.81 41.30 6.01 18.79 30.43
CV (%) 6.33 5.59 4.73 5.59 5.91 3.69

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