Methyl Antibodies | St John's Laboratory

Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H4-K5 Rabbit polyclonal antibody (STJ119872) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H4-K5 antibody (STJ119872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of 3T3 mouse-kidney KB K562 Hela 293T lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-Histone H4-Lys21 antibody (21 aa) (STJ98696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Mono-Methyl-Histone H4-Lys79 antibody (STJ97157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ11100898). Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H4-R3 antibody (STJ11100898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of HeLa cells using MonoMethyl-NONO/p54nrb-R357/R365 pAb (STJ110387) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-NONO-R357/R365 antibody (STJ110387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of MCF7 cells using Mono-Methyl-NF Kappa B-p65 (K314/K315) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-RELA-Lys314/Lys315 antibody (STJ90114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T COLO using TP53 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-TP53-Lys370 antibody (STJ97694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using P53 (Mono Methyl Lys370) Mouse mAb diluted at 1:500.

Anti-Mono-Methyl-TP53-Q59 antibody [Q59] (STJ97495)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody (STJ118448) at 1:1000 dilution.

Anti-Mono-Methyl-UHRF1-K385 antibody (STJ118448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using MonoMethyl-YAP1-K342 Rabbit polyclonal antibody (STJ11100581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Mono-Methyl-YAP1-K342 antibody (STJ11100581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of MonoMethyl-Histone H2B-K116 in paraffin-embedded rat heart using MonoMethyl-Histone H2B-K116 Rabbit monoclonal antibody (STJ11103791) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MonoMethyl-Histone H2B-K116 antibody [S3791RM] (STJ11103791)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H4-R3 Rabbit pAb (STJ11106422) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MonoMethyl-Histone H4-R3 antibody (STJ11106422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-MonoMethyl-PTEN-K349 antibody (STJ11106694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-MonoMethyl-PTEN-K349 antibody (STJ11106739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of Hela using Pan Methylated Lysine monoclonal antibody.

Anti-Pan-Methyl-Lysine-Mix antibody [Mix] (STJ97446)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Species independent

£48.00 - £252.00

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Symmetric DiMethyl-Histone H3-R8 antibody (STJ11103473) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Symmetric Di-Methyl-Histone H3-R8 antibody (STJ11103473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-Tri-Methyl-H3-Lys27 antibody (STJA0005911)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Tri-Methyl-H3-Lys37 antibody (STJA0005913)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Tri-Methyl-H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16-Lys21 antibody (STJA0005915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-Tri-Methyl-Histone H1-Lys25 antibody (STJ197301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H2B-Lys43 antibody (STJ197298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) Rat Brain, 3) 3T3, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H2B-Lys5 antibody (STJ197297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-Tri-Methyl-Histone H3-K10 antibody (10 aa) (STJ98695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K14 polyclonal antibody (STJ27232) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H3-K14 antibody (10-20) (STJ27232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K27 Rabbit polyclonal antibody antibody (STJ23989) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Tri-Methyl-Histone H3-K27 antibody (19-37) (STJ23989)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g TriMethyl-Histone H3-K27 mouse monoclonal antibody antibody (STJ118639) , along with a Goat Anti-mouse IgG (H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3-K27 in representative gene loci (MYOD1) , as shown in figure.

Anti-Tri-Methyl-Histone H3-K27 antibody [S9MM] (STJ118639)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Western blot analysis of various lysates, using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Tri-Methyl-Histone H3-K36 antibody (32-42) (STJ23992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and H3K36me3 rabbit monoclonal antibody (STJ11103285). The ChIP sequencing results indicate the enrichment pattern of H3K36me3 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Tri-Methyl-Histone H3-K36 antibody [S5MR] (STJ11103285)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IP/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Chromatin immunoprecipitation analysis of extracts of 293F cells, using TriMethyl-Histone H3-K4 Rabbit polyclonal antibody antibody (STJ23983) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Tri-Methyl-Histone H3-K4 antibody (2-19) (STJ23983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-Tri-Methyl-Histone H3-K5 antibody (STJ98848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K56 polyclonal antibody (STJ29403) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H3-K56 antibody (STJ29403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K64 Rabbit polyclonal antibody (STJ11100934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H3-K64 antibody (STJ11100934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

Choose Options

Anti-Tri-Methyl-Histone H3-K79 antibody (STJ23995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody (STJ23986) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Tri-Methyl-Histone H3-K9 antibody (STJ23986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of 1) Hela, 2) Rat Testis Tissue, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys14 antibody (STJ197290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys18 antibody (STJ197289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Xiao, Zhen, et al. "Targeting P16INK4A in uterine serous carcinoma through inhibition of histone demethylation. " Oncology reports 41. 5 (2019) : 2667-2678.

Anti-Tri-Methyl-Histone H3-Lys27 antibody (STJ197288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Liver Tissue, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys36 antibody (STJ197287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Hela, diluted at 1) 1:1000 , 2) 1:5000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys36 antibody [Q12] (STJ96990)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, 3) Raw264. 7, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys4 antibody (STJ197286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Tri-Methyl-Histone H3-Lys79 antibody (STJ197285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Hela, diluted at 1:500. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys79 antibody [1B7] (STJ96994)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 1) Hela, 2) Rat Testis tissue, 3) Raw264.7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Tri-Methyl-Histone H3-Lys79 antibody [2C3] (STJ97002)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Bek Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Tri-Methyl-Histone H3-Lys79 antibody [3G3] (STJ96993)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7, diluted at 1:1000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys9 antibody [2E11] (STJ97006)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Hela, diluted at 1) 1:2, 000, 2) 1:5, 000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys9 antibody [4H8] (STJ97011)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ23972). Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H4-K20 antibody (STJ23972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-Tri-Methyl-Histone H4-Lys20 antibody (STJ197281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options