Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using CD248 Rabbit polyclonal antibody (STJ114579) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD248 antibody (18-150) (STJ114579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of HepG2 cells using CD248 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD248 antibody (341-390 aa) (STJ96632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CD25/IL2RA antibody [143-13] (STJ16102559)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/FUNC/WB/IF/IHC

Reactivity :

Human

€359.38 - €1,655.63

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Anti-CD26 antibody [202-36] (STJ16102562)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human/Rat

€359.38 - €1,655.63

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Anti-CD26 antibody [202.36] (STJ16100026)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC-F/IF/WB

Reactivity :

Human/Rat

€471.88

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Anti-CD26 antibody [5+00E+08] (STJ16100784)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/ELISA/IP/WB

Reactivity :

Rat

€828.13

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Confocal imaging of human tonsil using CD27 Rabbit monoclonal antibody (STJ11101350, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.

Anti-CD27 antibody (1-100) [S0MR] (STJ11101350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

€161.88 - €718.13

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD27 antibody (1-80 aa) (STJ191672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-CD27 (PT1818) antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1: K562Predicted band size: 29kDaObserved band size: 40kDa

Anti-CD27 antibody (160-260) [PT1818] (STJA0006232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€60.00 - €315.00

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STJ73070 (0. 3µg/ml) staining of Human Spleen (A) , Tonsil (B) and Thymus (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD27 antibody (161-175) (STJ73070)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

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Western blot analysis of lysates from Mouse lung, using CD27 Rabbit polyclonal antibody (STJ118100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD27 antibody (20-191) (STJ118100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€140.63 - €655.63

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Western blot analysis of extracts of Raji cells, using CD27 antibody (STJ22984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD27 antibody (20-191) (STJ22984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-CD27 antibody [203-6] (STJ16101136)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

€359.38 - €1,655.63

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Flow cytometry:1X10^6 293F (Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (2 Mu g/mL, left) or CD271/NGFR Rabbit monoclonal antibody (STJ11105254, 2 Mu g/mL, right).

Anti-CD271 antibody (32-254) [S5254RM] (STJ11105254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse/Rat

€161.88 - €718.13

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD272 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-CD272 antibody (30-183 aa) [R09-4A1] (STJA0011098)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

€308.13

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Western blot analysis of lysates from Mouse thymus, using CD274 Rabbit monoclonal antibody (STJ11105258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD274 antibody (19-235) [S5258RM] (STJ11105258)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€161.88 - €718.13

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ113753) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (19-238) (STJ113753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using CD274 antibody (STJ11103333) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD274 antibody (19-239) (STJ11103333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of extracts of HepG2 cells, using PD-L1/CD274 antibody (STJ11104347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD274 antibody (190-290) (STJ11104347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunofluorescence analysis of paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ22985) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD274 antibody (190-290) (STJ22985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry of baboon spleen with Anti-CD274 primary antibody (STJ502399) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD274 antibody (80-130) (STJ502399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€534.38

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Anti-CD274 antibody (80-130) {Biotin} (STJ502400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€660.63

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Anti-CD274 antibody (80-130) {FITC} (STJ502401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€660.63

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274/CD274 Rabbit monoclonal antibody (STJ11103966) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (ECD) [S3966RM] (STJ11103966)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€161.88 - €718.13

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Anti-CD274 antibody (Extracellular region) [M051] (STJA0005523)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

€493.13

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Anti-CD274 antibody (Extracellular region) [M080] (STJA0005524)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

€493.13

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Anti-CD274 antibody [SMM] (STJ11106420)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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STJ70646 (0. 01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD274/PD-L1 antibody (C-Term) (STJ70646)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

€390.63

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Biotinylated STJ70646 (0. 3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-CD274/PD-L1, Biotinylated antibody (C-Term) {Biotin} (STJ73200)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

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Western blot analysis of Mouse spleen, using CD275 Rabbit polyclonal antibody (STJ11105078) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD275 antibody (47-270 aa) (STJ11105078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€140.63 - €655.63

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Western blot analysis of 293 cells using CD276 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD276 antibody (271-320 aa) (STJ96650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of CD276 in paraffin-embedded human thyroid cancer tissue using CD276 Rabbit polyclonal antibody (STJ11105431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CD276 antibody (29-245) (STJ11105431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of various lysates, using CD276/B7-H3 rabbit polyclonal antibody (STJ119347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : RajiExposure time: 180s.

Anti-CD276 antibody (29-245) (STJ119347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-CD276 antibody (Extracellular region) [M045] (STJA0005278)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human

€488.13

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Western blot analysis using CD276 monoclonal antibody against HEK293 (1) and CD276-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CD276 antibody [6A1] (STJ97917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

€180.00 - €435.00

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Western blot analysis of various lysates, using CD276/B7-H3 Rabbit monoclonal antibody (STJ11105227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : RajiExposure time: 90s.

Anti-CD276/B7-H3 antibody [S5227RM] (STJ11105227)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Anti-CD279/Pdcd1 antibody [29F.1A12] (STJA0001817)

Clonality :

Monoclonal

Host :

Rat

Applications :

Block/Neut/FC/WB/IHC/IF

Reactivity :

Mouse

€116.88 - €235.63

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Anti-CD279/Pdcd1 antibody [29F.1A12] (STJA0001818)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/WB/IHC/IF

Reactivity :

Mouse

€116.88 - €143.13

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Western blot analysis of K562 cells using CD28 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD28 antibody (11-60 aa) (STJ96609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Flow cytometric analysis of CD28 Rabbit monoclonal antibody Cocotail (10 Mu g/mL) in Jurkat cells (Orange) compare to Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) Isotype control (Blue) and non-staining control (Red).

Anti-CD28 antibody (Full Length) [S5MR] (STJ11103275)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Anti-CD28 antibody (Ig-V-like Region) (STJA0005685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€569.38

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STJ72503 (0. 3µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD28 antibody (Internal) (STJ72503)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

€390.63

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STJ72504 (0. 3µg/ml) staining of Mouse Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Cd28-Mouse antibody (Internal) (STJ72504)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse

€390.63

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Anti-CD29 antibody [MEM-101A] (STJ16100040)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Dog/Pig/Human

€506.88

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Anti-CD294 antibody (1-32 aa) (STJ11107024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Anti-CD298/ATP1B3 antibody (180-279 aa) [SRM] (STJ11106584)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD299/DC-SIGNR using CD299/DC-SIGNR Rabbit mAb (STJ11107384) at 1:3000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 20 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit ( Exposure time: 180s.

Anti-CD299/DC-SIGNR antibody (71-399 aa) [SRM] (STJ11107384)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

€161.88 - €718.13

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Anti-CD2AP antibody (250-300 aa) (STJ13100972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

€414.38

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