| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
NHERF-1 Positive Control for STJ506130 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
NHERF1 |
| Gene ID: |
9368 |
| Uniprot ID: |
NHRF1_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with NHERF-1 antibody STJ506130. |
| Tissue Specificity | Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast. |
| Post Translational Modifications | Phosphorylated on serine residues. |
| Function | Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli. Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. |
| Peptide Name | Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf1Nherf-1Ezrin-Radixin-Moesin-Binding Phosphoprotein 50Ebp50Regulatory Cofactor Of Na(+/H(+ ExchangerSodium-Hydrogen Exchanger Regulatory Factor 1Solute Carrier Family 9 Isoform A3 Regulatory Factor 1 |
| Cellular Localisation | CytoplasmApical Cell MembraneEndomembrane SystemPeripheral Membrane ProteinCell ProjectionFilopodiumRuffleMicrovillusTranslocates From The Cytoplasm To The Apical Cell Membrane In A Podxl-Dependent MannerColocalizes With Cftr At The Midpiece Of Sperm TailColocalizes With Actin In Microvilli-Rich Apical Regions Of The SyncytiotrophoblastFound In MicrovilliRuffling Membrane And Filopodia Of Hela CellsPresent In Lipid Rafts Of T-Cells |
| Alternative Peptide Names | Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf1 proteinNherf-1 proteinEzrin-Radixin-Moesin-Binding Phosphoprotein 50 proteinEbp50 proteinRegulatory Cofactor Of Na(+/H(+ Exchanger proteinSodium-Hydrogen Exchanger Regulatory Factor 1 proteinSolute Carrier Family 9 Isoform A3 Regulatory Factor 1 proteinNHERF1 proteinNHERF proteinSLC9A3R1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
