| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This TFR1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Tfrc |
| Gene ID: |
22042 |
| Uniprot ID: |
TFR1_MOUSE |
| Specificity: |
This kit recognizes Mouse TFR1 in samples. No significant cross-reactivity or interference between Mouse TFR1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Upon stimulation, positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2. |
| Protein Name | Transferrin Receptor Protein 1TrTfrTfr1TrfrCd Antigen Cd71 |
| Database Links | Reactome: R-MMU-432722Reactome: -MMU-8856825Reactome: -MMU-8856828Reactome: -MMU-8980692Reactome: -MMU-9013026Reactome: -MMU-9013106Reactome: -MMU-9013149Reactome: -MMU-9013404Reactome: -MMU-9013406Reactome: -MMU-9013407Reactome: -MMU-9013408Reactome: -MMU-9013423Reactome: -MMU-917977Reactome: -MMU-9696270Reactome: -MMU-9696273 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinMelanosome |
| Alternative ELISA Names | Transferrin Receptor Protein 1 ELISA kitTr ELISA kitTfr ELISA kitTfr1 ELISA kitTrfr ELISA kitCd Antigen Cd71 ELISA kitTfrc ELISA kitTrfr ELISA kit |
| Specificity | This kit recognizes Mouse TFR1 in samples. No significant cross-reactivity or interference between Mouse TFR1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-102 | 94 |
| EDTA plasma (n=8) | 95-106 | 101 |
| Cell culture media (n=8) | 85-97 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.84 | 3.55 | 18.61 | 1.66 | 3.50 | 19.61 |
| Standard deviation | 0.10 | 0.17 | 0.78 | 0.09 | 0.18 | 1.07 |
| CV (%) | 5.43 | 4.79 | 4.19 | 5.42 | 5.14 | 5.46 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
