| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.051ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This LPIN1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates or other biological fluids. |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Lpin1 |
| Gene ID: |
14245 |
| Uniprot ID: |
LPIN1_MOUSE |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Specifically expressed in skeletal muscle. Also expressed prominently in adipose tissue, and testis. Lower expression also detected in kidney, lung, brain and liver. Isoform 1: Predominant isoform in the liver. Isoform 2: Predominant isoform in the brain. |
| Post Translational Modifications | Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway. Phosphorylation is decreased by epinephrine. Phosphorylation may not directly affect the catalytic activity but may regulate the localization. Dephosphorylated by the CTDNEP1-CNEP1R1 complex. Phosphorylated at multiple sites by mTOR in response to insulin, leading to its inactivation. Phosphorylation does not affect the catalytic activity but regulates the localization. Phosphorylation is decreased by epinephrine. Dephosphorylated by the CTDNEP1-CNEP1R1 complex. Dephosphorylation following mTOR inhibition promotes its activity. Sumoylation is important in brain and is marginal in other tissues. Sumoylation facilitates nuclear localization of isoform 2 in neuronals cells and its transcriptional coactivator activity. Acetylation at Lys-459 and Lys-629 by KAT5 in response to fatty acids promotes translocation to the endoplasmic reticulum and synthesis of diacylglycerol. |
| Function | Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. Is involved in adipocyte differentiation. Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. Isoform 1: Recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol. |
| Protein Name | Phosphatidate Phosphatase Lpin1Fatty Liver Dystrophy ProteinLipin-1 |
| Database Links | Reactome: R-MMU-1483191Reactome: -MMU-1483213Reactome: -MMU-4419969Reactome: -MMU-75109 |
| Cellular Localisation | Isoform 1: Mitochondrion Outer MembraneCytoplasmNucleus MembraneRecruited At The Mitochondrion Outer Membrane Following Phosphatidic Acid Formation Mediated By Pld6In Neuronals CellsIsoform 1 Is Exclusively CytoplasmicIn 3t3-L1 Pre-AdipocytesIt Primarily Located In The CytoplasmIsoform 2: NucleusEndoplasmic Reticulum MembraneNuclear Localization Requires Both Cnep1r1 And Ctdnep1Localized In Both The Cytoplasm And The NucleusIt Is Predominantly NuclearTranslocates From The Cytosol To The Endoplasmic Reticulum Following Acetylation By Kat5 |
| Alternative ELISA Names | Phosphatidate Phosphatase Lpin1 ELISA kitFatty Liver Dystrophy Protein ELISA kitLipin-1 ELISA kitLpin1 ELISA kitFlde ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
