Mouse IGFBP-6 (Insulin-like Growth Factor Binding Protein 6) Sandwich ELISA Kit (STJE0019703)

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STJE0019703
£163.50 - £357.50
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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81-500pg/mL
Short Description : This IGFBP-6 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Igfbp6
Gene ID: 16012
Uniprot ID: IBP6_MOUSE
Specificity: This kit recognizes Mouse IGFBP-6 in samples. No significant cross-reactivity or interference between Mouse IGFBP-6 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Activates the MAPK signaling pathway and induces cell migration.
Protein Name Insulin-Like Growth Factor-Binding Protein 6
Ibp-6
Igf-Binding Protein 6
Igfbp-6
Database Links Reactome: R-MMU-381426
Cellular Localisation Secreted
Alternative ELISA Names Insulin-Like Growth Factor-Binding Protein 6 ELISA kit
Ibp-6 ELISA kit
Igf-Binding Protein 6 ELISA kit
Igfbp-6 ELISA kit
Igfbp6 ELISA kit
Igfbp-6 ELISA kit
Specificity This kit recognizes Mouse IGFBP-6 in samples. No significant cross-reactivity or interference between Mouse IGFBP-6 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 101
EDTA plasma (n=8) 94-108 100
Cell culture media (n=8) 87-101 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 25.57 50.36 243.90 25.46 49.57 266.77
Standard deviation 1.70 2.21 12.41 1.42 2.57 14.06
CV (%) 6.63 4.39 5.09 5.56 5.19 5.27

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