| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.073ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This GRIA4 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Gria4 |
| Gene ID: |
14802 |
| Uniprot ID: |
GRIA4_MOUSE |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Palmitoylated. Depalmitoylated upon L-glutamate stimulation. ZDHHC3/GODZ specifically palmitoylates Cys-611, which leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. Phosphorylated at Ser-862 by PRKCG.phosphorylation increases plasma membrane-associated GRI4 expression. |
| Function | Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate. |
| Protein Name | Glutamate Receptor 4Glur-4Glur4Ampa-Selective Glutamate Receptor 4Glur-DGlutamate Receptor Ionotropic - Ampa 4Glua4 |
| Database Links | Reactome: R-MMU-399710Reactome: -MMU-399719Reactome: -MMU-416993Reactome: -MMU-438066Reactome: -MMU-8849932 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinPostsynaptic Cell MembraneCell ProjectionDendrite |
| Alternative ELISA Names | Glutamate Receptor 4 ELISA kitGlur-4 ELISA kitGlur4 ELISA kitAmpa-Selective Glutamate Receptor 4 ELISA kitGlur-D ELISA kitGlutamate Receptor Ionotropic - Ampa 4 ELISA kitGlua4 ELISA kitGria4 ELISA kitGlur4 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
