| Function | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration. Plays a role in enhancing learning and memory performance. Plays a role in mammalian pain signaling (long-lasting hypersensitivity). |
| Protein Name | Epidermal Growth Factor Receptor |
| Database Links | Reactome: R-MMU-1227986Reactome: -MMU-1236394Reactome: -MMU-1250196Reactome: -MMU-1257604Reactome: -MMU-177929Reactome: -MMU-179812Reactome: -MMU-180292Reactome: -MMU-180336Reactome: -MMU-182971Reactome: -MMU-1963642Reactome: -MMU-212718Reactome: -MMU-2179392Reactome: -MMU-445144Reactome: -MMU-5673001Reactome: -MMU-6785631Reactome: -MMU-6811558Reactome: -MMU-8847993Reactome: -MMU-8856825Reactome: -MMU-8856828Reactome: -MMU-8857538Reactome: -MMU-8863795Reactome: -MMU-9009391Reactome: -MMU-9013507 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum MembraneGolgi Apparatus MembraneNucleus MembraneEndosomeEndosome MembraneNucleusIn Response To EgfTranslocated From The Cell Membrane To The Nucleus Via Golgi And ErEndocytosed Upon Activation By LigandColocalized With Gper1 In The Nucleus Of Estrogen Agonist-Induced Cancer-Associated Fibroblasts (Caf) |
| Alternative ELISA Names | Epidermal Growth Factor Receptor ELISA kitEgfr ELISA kit |
| Specificity | This kit recognizes Mouse EGFR in samples. No significant cross-reactivity or interference between Mouse EGFR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-102 | 96 |
| EDTA plasma (n=8) | 96-107 | 101 |
| Cell culture media (n=8) | 86-101 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 19.12 | 47.06 | 146.64 | 17.69 | 42.37 | 151.93 |
| Standard deviation | 1.08 | 2.43 | 4.78 | 0.89 | 1.86 | 6.41 |
| CV (%) | 5.66 | 5.16 | 3.26 | 5.02 | 4.40 | 4.22 |
