| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This mouse ASGR1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Asgr1 |
| Gene ID: |
11889 |
| Uniprot ID: |
ASGR1_MOUSE |
| Specificity: |
This kit recognizes Mouse ASGR1 in samples. No significant cross-reactivity or interference between Mouse ASGR1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed exclusively in hepatic parenchymal cells. |
| Post Translational Modifications | Phosphorylated on a cytoplasmic Ser residue. |
| Function | Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface. |
| Protein Name | Asialoglycoprotein Receptor 1Asgp-R 1Asgpr 1Hepatic Lectin 1Hl-1Mhl-1 |
| Database Links | Reactome: R-MMU-446203 |
| Cellular Localisation | MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative CLIA Names | Asialoglycoprotein Receptor 1 CLIA kitAsgp-R 1 CLIA kitAsgpr 1 CLIA kitHepatic Lectin 1 CLIA kitHl-1 CLIA kitMhl-1 CLIA kitAsgr1 CLIA kitAsgr-1 CLIA kit |
| Specificity | This kit recognizes Mouse ASGR1 in samples. No significant cross-reactivity or interference between Mouse ASGR1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-112 | 102 |
| EDTA plasma(n=8) | 96-110 | 103 |
| Cell culture media(n=8) | 94-108 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 37.97 | 109.64 | 386.71 | 35.55 | 105.79 | 415.65 |
| Standard deviation | 3.71 | 9.76 | 39.83 | 4.59 | 12.42 | 28.39 |
| CV (%) | 9.77 | 8.90 | 10.30 | 12.91 | 11.74 | 6.83 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
