| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 28pg/mL |
| Detection Limit: | 78.1-5000pg/mL |
| Short Description : | This TLR3 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | TLR3 |
| Gene ID: | 7098 |
| Uniprot ID: | TLR3_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. |
| Post Translational Modifications | Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. TLR3 signaling requires a proteolytic cleavage mediated by cathepsins CTSB and CTSH, the cleavage occurs between amino acids 252 and 346. The cleaved form of TLR3 is the predominant form found in endosomes. Ubiquitinated by TRIM3.leading to recognition and sorting of polyubiquitinated TLR3 by the ESCRT complexes. Ubiquitinated by ZNRF1 via 'Lys-63'-linked ubiquitin chains.leading to TLR3 lysosomal trafficking and degradation. Ubiquitinated by RNF170 at Lys-765 via 'Lys-48'-linked ubiquitin chains.leading to TLR3 proteasomal degradation. |
| Function | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response. |
| Protein Name | Toll-Like Receptor 3 Cd Antigen Cd283 |
| Database Links | Reactome: R-HSA-1679131 Reactome: R-HSA-168164 Reactome: R-HSA-168927 Reactome: R-HSA-1810476 Reactome: R-HSA-5602410 Reactome: R-HSA-5602415 Reactome: R-HSA-5602566 Reactome: R-HSA-5602571 Reactome: R-HSA-9013957 Reactome: R-HSA-9013973 Reactome: R-HSA-9014325 Reactome: R-HSA-9828211 Reactome: R-HSA-9833110 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Type I Membrane Protein Endosome Membrane Early Endosome |
| Alternative ELISA Names | Toll-Like Receptor 3 ELISA kit Cd Antigen Cd283 ELISA kit TLR3 ELISA kit |
| output |
Information sourced from Uniprot.org