| Function | Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes).specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S).the function is different from the established EJC assembly. |
| Protein Name | Rna-Binding Protein 8aBinder Of Ovca1-1Bov-1Rna-Binding Motif Protein 8aRna-Binding Protein Y14Ribonucleoprotein Rbm8a |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856Reactome: R-HSA-9010553Reactome: R-HSA-975957 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmNucleocytoplasmic Shuttling ProteinTravels To The Cytoplasm As Part Of The Exon Junction Complex (Ejc) Bound To MrnaColocalizes With The Core EjcAlyref/Thoc4Nxf1 And Uap56 In The Nucleus And Nuclear Speckles |
| Alternative Protein Names | Rna-Binding Protein 8a proteinBinder Of Ovca1-1 proteinBov-1 proteinRna-Binding Motif Protein 8a proteinRna-Binding Protein Y14 proteinRibonucleoprotein Rbm8a proteinRBM8A proteinRBM8 proteinHSPC114 proteinMDS014 protein |
Information sourced from Uniprot.org
