• Human PRF1 (Perforin 1) Sandwich ELISA Kit (STJE0002736)

Human PRF1 (Perforin 1) Sandwich ELISA Kit (STJE0002736)

SKU:
STJE0002736

Current Stock:
Applications: ELISA
Reactivity: Human
Note: FOR RESEARCH USE ONLY (RUO).
Sensitivity: 0.47 ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This PRF1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Detection: Colorimetric
Gene Symbol: PRF1
Gene ID: 5551
Uniprot ID: PERF_HUMAN
Sample Type: serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated.
Function Pore-forming protein that plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Protein Name Perforin-1
P1
Cytolysin
Lymphocyte Pore-Forming Protein
Pfp
Cellular Localisation Cytolytic Granule
Secreted
Cell Membrane
Multi-Pass Membrane Protein
Endosome Lumen
Stored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target Cell
Inserts Into The Cell Membrane Of Target Cells And Forms Pores
Membrane Insertion And Pore Formation Requires A Major Conformation Change
May Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes
Alternative Antibody Names Anti-Perforin-1 antibody
Anti-P1 antibody
Anti-Cytolysin antibody
Anti-Lymphocyte Pore-Forming Protein antibody
Anti-Pfp antibody
Anti-PRF1 antibody
Anti-PFP antibody
Specificity This kit recognizes Human PRF1 in samples. No significant cross-reactivity or interference between Human PRF1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma (n=8) 88-102 95
Cell culture media (n=8) 94-112 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.70 6.61 23.43 2.46 7.19 21.35
Standard deviation 0.18 0.30 0.98 0.17 0.42 1.06
CV (%) 6.67 4.54 4.18 6.91 5.84 4.96
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance