| Host: | Goat/Goat |
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description : | This IL-6R antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Clonality : | Polyclonal/Polyclonal |
| Conjugation: | Unconjugated/Biotin |
| Formulation: | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Purification: | Capture Purification: Antigen Affinity. Detection Purification: Antigen Affinity. |
| Concentration: | 0.5 mg/mL |
| Storage Instruction: | Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Gene Symbol: | IL6R |
| Gene ID: | 3570 |
| Uniprot ID: | IL6RA_HUMAN |
| Immunogen: | Capture immunogen: Recombinant Human IL-6R protein. Detection Immunogen: Recombinant Human IL-6R protein. |
| Post Translational Modifications | A short soluble form is released from the membrane by proteolysis. The sIL6R is formed mostly by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding, but is also directly secreted from the cells after alternative mRNA splicing. mIL6R is cleaved by the proteases ADAM10 and ADAM17. Glycosylated. Glycosylation is dispensable for transport, signaling, and cell-surface turnover. Glycosylation at Asn-55 is a protease-regulatory exosite. Glycosylation is required for ADAM17-mediated proteolysis. |
| Function | Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). Isoform 1: Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Isoform 2: Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity. The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases. In complex with IL6, is required for induction of VEGF production. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. Soluble interleukin-6 receptor subunit alpha: Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity. The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases. In complex with IL6, is required for induction of VEGF production. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. |
| Protein Name | Interleukin-6 Receptor Subunit Alpha Il-6 Receptor Subunit Alpha Il-6r Subunit Alpha Il-6r-Alpha Il-6ra Il-6r 1 Membrane Glycoprotein 80 Gp80 Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit Alpha Sil6r |
| Database Links | Reactome: R-HSA-1059683 Reactome: R-HSA-110056 Reactome: R-HSA-112411 Reactome: R-HSA-6785807 Reactome: R-HSA-9616222 Reactome: R-HSA-9679191 |
| Cellular Localisation | Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Isoform 2: Secreted Soluble Interleukin-6 Receptor Subunit Alpha: Secreted |
| Alternative Antibody Pair Names | Interleukin-6 Receptor Subunit Alpha antibody pair Il-6 Receptor Subunit Alpha antibody pair Il-6r Subunit Alpha antibody pair Il-6r-Alpha antibody pair Il-6ra antibody pair Il-6r 1 antibody pair Membrane Glycoprotein 80 antibody pair Gp80 antibody pair Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit Alpha antibody pair Sil6r antibody pair IL6R antibody pair |
Information sourced from Uniprot.org