| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% Trehalose is added as protectant before lyophilisation. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <1 EU/Mu g of the protein by LAL method |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Human IGHA1 at 5 Mu g/mL (100 Mu L/well) can bind Human CD89/FCAR with a linear range of 0.01-7.4 ng/mL. |
| Immunogen Region: | Cys122-Tyr353 |
| Immunogen: | Recombinant Recombinant Human IgA1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys122-Tyr353) of Human IgA1 (Accession #P01876-1) fused with His tag at the N-terminus. |
| Immunogen Sequence: | LDKYGVSDYHKNLINNAKTV EGVKDLQAQVVESAKKARIS EATDGLSDFLKSQTPAEDTV KSIELAEAKVLANRELDKYG VSDYYKNLINNAKTVEGVKA LIDEILAALPKTDTYKLILN GKTLKGETTTEAVDAATAEK VFKQYANDNGVDGEWTYDDA TKTFTVTEKPEVIDASELTP AVTTYKLVINGKTLK |
| Background | Recombinant Human IgA1 Protein, Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens.The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V- (D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. |
Information sourced from Uniprot.org
