| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human Her2/ErbB2-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Conjugation: | Biotin |
| Formulation: | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Storage Instruction: | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: | ERBB2 |
| Gene ID: | 2064 |
| Uniprot ID: | ERBB2_HUMAN |
| Immunogen Region: | Thr23-Thr652 |
| Immunogen: | DNA sequence encoding Human Her2/ErbB2 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Post Translational Modifications | Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. Dephosphorylated by PTPN12. |
| Function | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A.the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. |
| Protein Name | Receptor Tyrosine-Protein Kinase Erbb-2 Metastatic Lymph Node Gene 19 Protein Mln 19 Proto-Oncogene Neu Proto-Oncogene C-Erbb-2 Tyrosine Kinase-Type Cell Surface Receptor Her2 P185erbb2 Cd Antigen Cd340 |
| Database Links | Reactome: R-HSA-1227986 Reactome: R-HSA-1250196 Reactome: R-HSA-1251932 Reactome: R-HSA-1257604 Reactome: R-HSA-1306955 Reactome: R-HSA-1358803 Reactome: R-HSA-1963640 Reactome: R-HSA-1963642 Reactome: R-HSA-2219530 Reactome: R-HSA-416572 Reactome: R-HSA-5673001 Reactome: R-HSA-6785631 Reactome: R-HSA-6811558 Reactome: R-HSA-8847993 Reactome: R-HSA-8863795 Reactome: R-HSA-8866910 Reactome: R-HSA-9634285 Reactome: R-HSA-9652282 Reactome: R-HSA-9664565 Reactome: R-HSA-9665233 Reactome: R-HSA-9665244 Reactome: R-HSA-9665245 Reactome: R-HSA-9665246 Reactome: R-HSA-9665247 Reactome: R-HSA-9665249 Reactome: R-HSA-9665250 Reactome: R-HSA-9665251 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686 Reactome: R-HSA-9665737 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Ruffle Membrane Internalized From The Cell Membrane In Response To Egf Stimulation Isoform 1: Cell Membrane Early Endosome Cytoplasm Perinuclear Region Nucleus Translocation To The Nucleus Requires Endocytosis Probably Endosomal Sorting And Is Mediated By Importin Beta-1/Kpnb1 Also Detected In Vps35-Positive Endosome-To-Tgn Retrograde Vesicles Isoform 2: Cytoplasm Isoform 3: Cytoplasm |
| Alternative Protein Names | Receptor Tyrosine-Protein Kinase Erbb-2 protein Metastatic Lymph Node Gene 19 Protein protein Mln 19 protein Proto-Oncogene Neu protein Proto-Oncogene C-Erbb-2 protein Tyrosine Kinase-Type Cell Surface Receptor Her2 protein P185erbb2 protein Cd Antigen Cd340 protein ERBB2 protein HER2 protein MLN19 protein NEU protein NGL protein |
Information sourced from Uniprot.org