| Post Translational Modifications | Polyubiquitinated by RNF217.leading to proteasomal degradation. Under conditions of high systemic iron levels, both the hormone peptide hepcidin/HAMP and holo(iron bound)-transferrin/TF induce the ubiquitination, internalization and proteasomal degradation of SLC40A1 to control iron release from cells. |
| Function | Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma. |
| Protein Name | FerroportinFerroportin-1Iron-Regulated Transporter 1Solute Carrier Family 40 Member 1 |
| Database Links | Reactome: R-HSA-425410Reactome: R-HSA-5619049Reactome: R-HSA-5619060Reactome: R-HSA-5655799Reactome: R-HSA-917937 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneLocalized To The Basolateral Membrane Of Hepatocytoma Wif-B Cells |
| Alternative ELISA Names | Ferroportin ELISA kitFerroportin-1 ELISA kitIron-Regulated Transporter 1 ELISA kitSolute Carrier Family 40 Member 1 ELISA kitSLC40A1 ELISA kitFPN ELISA kitFPN1 ELISA kitIREG1 ELISA kitSLC11A3 ELISA kitMSTP079 ELISA kit |
| Specificity | This kit recognizes Human FPN in samples. No significant cross-reactivity or interference between Human FPN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-96 | 90 |
| EDTA plasma (n=8) | 93-104 | 99 |
| Cell culture media (n=8) | 90-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.92 | 2.31 | 7.33 | 0.98 | 2.22 | 6.66 |
| Standard deviation | 0.05 | 0.12 | 0.23 | 0.06 | 0.10 | 0.29 |
| CV (%) | 5.43 | 5.19 | 3.14 | 6.12 | 4.50 | 4.35 |
