| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human ERCC3-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | ERCC3 |
| Gene ID: | 2071 |
| Uniprot ID: | ERCC3_HUMAN |
| Immunogen Region: | Arg542-Ala702 |
| Immunogen: | Homo sapiens (Human) |
| Function | ATP-dependent 3'-5' DNA helicase/translocase. Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex. When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening.it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. In transcription pre-initiation complexes induces and propagates a DNA twist to open DNA. Also involved in transcription-coupled nucleotide excision repair (NER) of damaged DNA. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex.large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA. XPA retains XPB/ERCC3 at the 5' end of a DNA bubble (mimicking DNA damage). |
| Protein Name | General Transcription And Dna Repair Factor Iih Helicase/Translocase Subunit XpbTfiih Subunit XpbBasic Transcription Factor 2 89 Kda SubunitBtf2 P89Dna 3'-5' Helicase/Translocase XpbDna Excision Repair Protein Ercc-3Dna Repair Protein Complementing Xp-B CellsTfiih Basal Transcription Factor Complex 89 Kda SubunitTfiih 89 Kda SubunitTfiih P89Xeroderma Pigmentosum Group B-Complementing Protein |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | General Transcription And Dna Repair Factor Iih Helicase/Translocase Subunit Xpb proteinTfiih Subunit Xpb proteinBasic Transcription Factor 2 89 Kda Subunit proteinBtf2 P89 proteinDna 3'-5' Helicase/Translocase Xpb proteinDna Excision Repair Protein Ercc-3 proteinDna Repair Protein Complementing Xp-B Cells proteinTfiih Basal Transcription Factor Complex 89 Kda Subunit proteinTfiih 89 Kda Subunit proteinTfiih P89 proteinXeroderma Pigmentosum Group B-Complementing Protein proteinERCC3 proteinXPB proteinXPBC protein |
Information sourced from Uniprot.org